Effect of CDP-choline on age-dependent modifications of energy- and glutamate-linked enzyme activities in synaptic and non-synaptic mitochondria from rat cerebral cortex
| Autor: | Antonella Gorini, Federica Ferrari, Roberto Federico Villa |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Aging Cytidine Diphosphate Choline Citric Acid Cycle Glutamic Acid Nerve Tissue Proteins Dehydrogenase Mitochondrion Malate dehydrogenase Catalysis Random Allocation Cellular and Molecular Neuroscience medicine Animals Citrate synthase Rats Wistar Neurons chemistry.chemical_classification biology Glutamate dehydrogenase Glutamate receptor Cell Biology Enzymes Frontal Lobe Mitochondria Rats Enzyme Activation Neuroprotective Agents medicine.anatomical_structure Enzyme Biochemistry chemistry Cerebral cortex biology.protein Synaptosomes |
| Zdroj: | Neurochemistry International. 61:1424-1432 |
| ISSN: | 0197-0186 |
| DOI: | 10.1016/j.neuint.2012.10.009 |
| Popis: | The effect of aging and CDP-choline treatment (20 mg kg−1 body weight i.p. for 28 days) on the maximal rates (Vmax) of representative mitochondrial enzyme activities related to Krebs’ cycle (citrate synthase, α-ketoglutarate dehydrogenase, malate dehydrogenase), glutamate and related amino acid metabolism (glutamate dehydrogenase, glutamate–oxaloacetate- and glutamate–pyruvate transaminases) were evaluated in non-synaptic and intra-synaptic “light” and “heavy” mitochondria from frontal cerebral cortex of male Wistar rats aged 4, 12, 18 and 24 months. During aging, enzyme activities vary in a complex way respect to the type of mitochondria, i.e. non-synaptic and intra-synaptic. This micro-heterogeneity is an important factor, because energy-related mitochondrial enzyme catalytic properties cause metabolic modifications of physiopathological significance in cerebral tissue in vivo, also discriminating pre- and post-synaptic sites of action for drugs and affecting tissue responsiveness to noxious stimuli. Results show that CDP-choline in vivo treatment enhances cerebral energy metabolism selectively at 18 months, specifically modifying enzyme catalytic activities in non-synaptic and intra-synaptic “light” mitochondrial sub-populations. This confirms that the observed changes in enzyme catalytic activities during aging reflect the bioenergetic state at each single age and the corresponding energy requirements, further proving that in vivo drug treatment is able to interfere with the neuronal energy metabolism. |
| Databáze: | OpenAIRE |
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