Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas
Autor: | Chiaki Kato, Satoshi Wakai, Yoshihiro Sambongi, Shinya Kobayashi, Masayoshi Nishiyama, Yoshie Harada, Sotaro Fujii, Misa Masanari-Fujii |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Chemistry Cytochrome c Organic Chemistry Pseudomonas General Medicine biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Deep sea Shewanella Analytical Chemistry 03 medical and health sciences 030104 developmental biology Protein stability biology.protein Molecular Biology Biotechnology |
DOI: | 10.6084/m9.figshare.5986732 |
Popis: | Two cytochromes c5 (SBcytc and SVcytc) have been derived from Shewanella living in the deep-sea, which is a high pressure environment, so it could be that these proteins are more stable at high pressure than at atmospheric pressure, 0.1 MPa. This study, however, revealed that SBcytc and SVcytc were more stable at 0.1 MPa than at higher pressure. In addition, at 0.1–150 MPa, the stability of SBcytc and SVcytc was higher than that of homologues from atmospheric-pressure Shewanella, which was due to hydrogen bond formation with the heme in the former two proteins. This study further revealed that cytochrome c551 (PMcytc) of deep-sea Pseudomonas was more stable than a homologue of atmospheric-pressure Pseudomonas aeruginosa, and that specific hydrogen bond formation with the heme also occurred in the former. Although SBcytc and SVcytc, and PMcytc are phylogenetically very distant, these deep-sea cytochromes c are commonly stabilized through hydrogen bond formation. Deep-sea cytochromes c are commonly stabilized through hydrogen bond formation. |
Databáze: | OpenAIRE |
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