Cell–cell communication induces random spikes of spontaneous calcium oscillations in multi-BV-2 microglial cells

Autor: Xian Wu, Imshik Lee, Jingjun Xu, Xinzheng Zhang, Ying Liu, Leiting Pan, Drevensek-Olenik Irena, Pengchong Jiang
Rok vydání: 2013
Předmět:
Zdroj: Biochemical and Biophysical Research Communications. 431:664-669
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2013.01.064
Popis: As the first and main form of active immune defense in the central nervous system, microglial cells usually exhibit complicated intracellular calcium (Ca²⁺) activity that can regulate the downstream components of signaling cascades. In the present work, spontaneous oscillations of the cytosolic calcium concentration ([Ca²⁺]c) in multi-BV-2 microglial cells were observed by video microscopy. These cells exhibited random spikes of Ca²⁺ oscillations. Cross-correlation analysis of the temporal dependence of the oscillations indicated the existence of cell-cell communication mediated by extracellular messengers. Numerical simulations based on a simple mathematical model suggested that these communications could induce random spikes of spontaneous Ca oscillations in the multi-cell system. Short-time imaging analysis of random spikes in different regions of a single cell showed that spontaneous Ca²⁺ oscillations resulted from Ca²⁺ wave generated by other cells as well as from calcium elevation inside the cell. Taken together, our data demonstrate that cell-cell communication existed between the BV-2 microglial cells in vitro and further resulted in the random spikes of spontaneous Ca²⁺ oscillations.
Databáze: OpenAIRE
Externí odkaz: