Males and females exhibit distinct relationships between intervertebral disc degeneration and pain in a rat model
| Autor: | Alon Lai, Grace E. Mosley, James C. Iatridis, Minghui Wang, Bin Zhang, Daniel A Charen, Philip Nasser |
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| Rok vydání: | 2020 |
| Předmět: |
Male
musculoskeletal diseases 0301 basic medicine Nervous system Pathology medicine.medical_specialty Rat model lcsh:Medicine Chronic pain Intervertebral Disc Degeneration Degeneration (medical) Article Rats Sprague-Dawley 03 medical and health sciences Sex Factors 0302 clinical medicine Lumbar Ganglia Spinal medicine Back pain Animals lcsh:Science Musculoskeletal system Lumbar Vertebrae Multidisciplinary business.industry lcsh:R Sham surgery Intervertebral disc musculoskeletal system Rats Disease Models Animal 030104 developmental biology Nociception medicine.anatomical_structure Back Pain Hyperalgesia Female lcsh:Q Gene expression Peripheral nervous system medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Back pain is linked to intervertebral disc (IVD) degeneration, but clinical studies show the relationship is complex. This study assessed whether males and females have distinct relationships between IVD degeneration and pain using an in vivo rat model. Forty-eight male and female Sprague–Dawley rats had lumbar IVD puncture or sham surgery. Six weeks after surgery, IVDs were evaluated by radiologic IVD height, histological grading, and biomechanical testing. Pain was assessed by von Frey assay and dorsal root ganglia (DRG) expression of Calca and Tac1 genes. Network analysis visualized which measures of IVD degeneration most related to pain by sex. In both females and males, annular puncture induced structural IVD degeneration, but functional biomechanical properties were similar to sham. Females and males had distinct differences in mechanical allodynia and DRG gene expression, even though sex differences in IVD measurements were limited. Network analysis also differed by sex, with more associations between annular puncture injury and pain in the male network. Sex differences exist in the interactions between IVD degeneration and pain. Limited correlation between measures of pain and IVD degeneration highlights the need to evaluate pain or nociception in IVD degeneration models to better understand nervous system involvement in discogenic pain. |
| Databáze: | OpenAIRE |
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