Different roles for the Fc epsilon RI gamma chain as a function of the receptor context
| Autor: | Rossella Paolini, Eric Vivier, V Renard, Bernard Malissen, Kenichi Ochiai, M.-H. Jouvin, Jean-Pierre Kinet |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Interleukin 2
Macromolecular Substances Receptors Antigen T-Cell alpha-beta CD3 T cell Molecular Sequence Data Immunology In Vitro Techniques Biology Transfection CD3 Complex Mice medicine Animals Immunology and Allergy Electrophoresis Gel Two-Dimensional Amino Acid Sequence Phosphotyrosine Receptor Receptors IgE T-cell receptor Articles Phosphoproteins Molecular biology medicine.anatomical_structure biology.protein Interleukin-2 Tyrosine Phosphorylation Signal transduction Signal Transduction medicine.drug |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.181.1.247 |
| Popis: | The high affinity immunoglobulin E receptor (Fc epsilon RI) and the B and T cell antigen receptors (TCR) are multimeric complexes containing subunits with cytoplasmic antigen recognition activation motifs (ARAMs). The presence of multiple motifs may be a way to amplify a single signal or provide independent activation modules. Here we have compared the signaling capacity of the same Fc epsilon RI gamma motif in the context of two different receptors, Fc epsilon RI and TCR/CD3, simultaneously reconstituted on the surface of the same zeta-deficient T cell line. Both reconstituted receptors mediate early (phosphorylation) and late (interleukin [IL]-2 release) signals. Mutation of the two tyrosine residues of ARAM gamma alters early signaling by both receptors, but the set of substrates phosphorylated via ARAM gamma is different for each receptor and is thus dependent on the receptor context. Furthermore, the mutations prevent Fc epsilon RI- but not TCR/CD3-mediated IL-2 release. These data demonstrate that ARAM gamma is necessary for allowing both receptors to phosphorylate the complete set of substrates, and that the CD3 complex, unlike the Fc epsilon RI beta chain, contains activation modules capable of compensating for the absence of a functional ARAM gamma in generating late signals such as IL-2 release. |
| Databáze: | OpenAIRE |
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