Effects of interleukin-2 on the pulmonary microvasculature in anesthetized sheep
| Autor: | Norman C. Staub, D. Lei, C. W. Johnson, D. Douguet, G. J. Jesmok, E. H. Jerome, D. P. Schuster |
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| Rok vydání: | 1994 |
| Předmět: |
Pulmonary Circulation
Pathology medicine.medical_specialty Endothelium Physiology Hemodynamics Thoracic duct Thoracic Duct Microcirculation Leukocyte Count Physiology (medical) medicine Animals Splanchnic Circulation Respiratory system Lung Blood Cells Sheep business.industry Osmolar Concentration medicine.anatomical_structure Extravascular Lung Water Interleukin-2 Lymph business Nuclear medicine Splanchnic |
| Zdroj: | Journal of Applied Physiology. 76:909-915 |
| ISSN: | 1522-1601 8750-7587 |
| Popis: | Interleukin-2 (IL-2) is reputed to cause pulmonary microvascular injury. We studied the pulmonary and splanchnic microcirculation of anesthetized sheep after one dose (1.8 x 10(6) IU/kg) of IL-2 (n = 9) and after six doses (1.8 x 10(6) IU.kg-1.dose-1) of IL-2 over 3 days (n = 9). Seven control sheep received only 5% dextrose diluent. We measured hemodynamics and lymph dynamics in anesthetized sheep after the final dose of IL-2 or diluent. After one dose of IL-2, caudal mediastinal node (mainly pulmonary) lymph flow was stable, whereas thoracic duct lymph flow increased from a baseline of 54 +/- 6 to 124 +/- 22 ml/h. After 3 days of IL-2, the caudal mediastinal node lymph flow increased from 7.7 +/- 5.5 to 19.0 +/- 14.8 ml/h 5–6 h after the final dose of IL-2, and thoracic duct lymph flow increased from 84 +/- 43 to 143 +/- 42 ml/h. The lymph-to-plasma protein concentration ratio increased after IL-2 for thoracic duct but not for caudal mediastinal node lymph. The equilibration rate of 125I-albumin from plasma to caudal mediastinal node lymph did not change, whereas plasma-to-thoracic duct lymph equilibration was faster after both one dose and 3 days of IL-2. Positron emission tomography showed no increase in the pulmonary transcapillary escape rate for 68Ga-labeled transferrin or in extravascular lung water (n = 4). We conclude that IL-2 at doses two to three times those used clinically does not significantly injure the pulmonary microcirculation of sheep. |
| Databáze: | OpenAIRE |
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