The role of pulmonary ORCC and CLC‑2 channels in the response to oxidative stress
| Autor: | Anna Guiotto, Marta Martini, Mascia Benedusi, Rita Canella, Giuseppe Valacchi, Franco Cervellati |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Negative voltage Health Toxicology and Mutagenesis Cell Pharmacology toxicology Toxicology medicine.disease_cause Pathology and Forensic Medicine Human lung NO Chloride channels Human lung epithelial cells Oxidative stress Ozone Patch clamp technique 03 medical and health sciences 0302 clinical medicine medicine General Pharmacology Toxicology and Pharmaceutics Chemistry Public Health Environmental and Occupational Health Membrane current 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Biophysics |
| Popis: | Background Exposure of human lung epithelial cells to the oxidant pollutant ozone (O3) alters cell Cl− currents inducing an outward rectifier effect. Among the various Cl− channels, ClC-2 and ORCC seemed to be involved in this response. Objectives To identify the channel related to O3 induced current changes. Results Down regulating the expression of ORCC and ClC-2 genes and analyzing the membrane current show that the enhancement of the current disappeared when ORCC was silenced. The contribution of ORCC and ClC-2 channels in control and O3 treated cells was obtained by a mathematical approach. Conclusion We suggest that O3 activates ORCC channels and slightly inhibited ClC-2 channels in the negative voltage range. These findings open the possibility of identifying the biomolecular changes induced by O3 allowing a possible pharmacological intervention towards chloride current due to oxidative stress. |
| Databáze: | OpenAIRE |
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