Involvement of SHV-12 and SHV-2a Encoding Plasmids in Outbreaks of Extended-Spectrum β-Lactamase-ProducingKlebsiella pneumoniaein a Tunisian Neonatal Ward
| Autor: | Thouraya Ben-Hamouda, Thierry Foulon, Kamel Ben-Mahrez |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Tunisia medicine.drug_class Klebsiella pneumoniae Immunology Cephalosporin Biology Polymerase Chain Reaction Microbiology beta-Lactamases Disease Outbreaks law.invention Plasmid law Patients' Rooms Genotype polycyclic compounds medicine Humans Gene Polymerase chain reaction DNA Primers Pharmacology Base Sequence Infant Newborn Nucleic acid sequence biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Klebsiella Infections Kinetics Transformation (genetics) Conjugation Genetic bacteria Plasmids |
| Zdroj: | Microbial Drug Resistance. 10:132-138 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/1076629041310118 |
| Popis: | Previous genotypic investigations of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae recovered in a Tunisian neonatal ward revealed the spread of two epidemic strains and a high number of genetically unrelated isolates. The aim of the present study was to determine the role of the dissemination of self-transferrable plasmids harboring bla genes in the outbreaks experienced by the ward. The 49 previously identified clinical isolates of ESBL-producing K. pneumoniae were examined for relationships between their enzymes and plasmids. Analysis of crude extracts by isoelectric focusing showed four beta-lactamase-activities at pI 8.2, 7.6, 6, and 5.4. Clinical isolates contained large plasmids that could be transferred by conjugation and transformation conferring resistance to expanded-spectrum cephalosporins. DNA amplification and sequencing were performed to confirm the identities of transferred beta-lactamases. Nucleotide sequence analysis of SHV-specific PCR products from six isolates identified two bla(SHV) genes corresponding to SHV derived ESBLs, SHV-12 and SHV-2a. PstI digestion of plasmid DNA from transformants revealed six restriction patterns. The occurrence of the prevalent plasmid pattern in both epidemic strains and unrelated isolates indicated that diffusion and endemic persistence of the bla(SHV-ESBL) genes in the ward were due to concomitant spread of epidemic strains and plasmid dissemination among unrelated strains. |
| Databáze: | OpenAIRE |
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