A sensitive and rapid immunochemical method for quantitation of proteins
| Autor: | Prabhakar K. Ranjekar, S. N. Ranadive, Madhumalti R. Mawal, Yogesh R. Mawal |
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| Rok vydání: | 1988 |
| Předmět: |
Globulin
Biophysics Enzyme-Linked Immunosorbent Assay Biochemistry Cajanus chemistry.chemical_compound Storage protein Molecular Biology chemistry.chemical_classification Plants Medicinal Chromatography biology food and beverages Fabaceae Globulins Cell Biology biology.organism_classification Enzyme Dextran chemistry Seeds biology.protein Antibody Peroxidase Conjugate |
| Zdroj: | Bioscience Reports. 8:427-433 |
| ISSN: | 1573-4935 0144-8463 |
| DOI: | 10.1007/bf01121640 |
| Popis: | A sensitive and rapid ELISA for quantitation of seed globulins is described. This method employs conjugation of pigeon pea (Cajanus cajan) globulin antibodies and the enzyme peroxidase together with dextran. Using this conjugate, proteins as low as 0.1 ng were detected. Dextran conjugate has a ten-fold greater efficiency of quantitating pigeon pea globulins than the commercial goat anti-rabbit IgG conjugate, and is three-fold more efficient than pigeon pea globulin IgG peroxidase conjugate. The method can be conveniently adapted for quantitation of other proteins also. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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