No major role for the transcription factor NF-κB in bone marrow function during peritonitis in the mouse
| Autor: | Harald Carlsen, Haakon B. Benestad, Per Ole Iversen, Eirunn Knudsen, Arne Bøyum |
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| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Lipopolysaccharide Neutrophils Gene Expression Mice Transgenic Inflammation Spleen Peritonitis Biology Mice chemistry.chemical_compound Peritoneal cavity Bone Marrow Genes Reporter Internal medicine medicine Animals Luciferases Lung Hematology Zymosan NF-kappa B Molecular biology Haematopoiesis medicine.anatomical_structure Liver Neutrophil Infiltration chemistry Peptones Immunology Macrophages Peritoneal Female Bone marrow medicine.symptom |
| Zdroj: | International Journal of Hematology. 100:111-118 |
| ISSN: | 1865-3774 0925-5710 |
| Popis: | Nuclear factor-kappa B (NF-κB) is a multipotent transcription factor that plays a pivotal role in immune reactions, inflammation, and possibly hematopoiesis as well. Mobilization of neutrophilic granulocytes during inflammation is a highly regulated process, but one that is incompletely understood. We studied the in vivo activity of NF-κB in mouse organs and cells, with a focus on bone marrow, during acute inflammation. NF-κB activity was studied in transgenic mice expressing a luciferase reporter expressed in a NF-κB activation-dependent fashion. Acute peritoneal inflammation was induced by lipopolysaccharide (LPS), the casein digest bacto-tryptone, or the insoluble polysaccharide zymosan. Organs were removed and blood, bone marrow, and peritoneal cells were separated using density gradient centrifugation. NF-κB activity in organ homogenates and cell lysates was quantified. These three inflammatory agents increased NF-κB activity to a variable extent within the inflamed peritoneal cavity, liver, and spleen, with LPS being the strongest stimulus. LPS, but not bacto-tryptone or zymosan, activated NF-κB in lung and bone marrow, the latter activity mainly observed in density fractions rich in immature bone marrow cells. NF-κB activation was prominent at 6 h after induction of peritonitis, fading at 24 h, as expected for an acute phase phenomenon. From this proof-of-principle study with luciferase reporter mice dependent on NF-κB activation, we suggest that, in steady-state mice, mobilization of bone marrow granulocytes to an inflammatory site can occur without discernible activation of NF-κB in bone marrow. |
| Databáze: | OpenAIRE |
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