Genome resources for climate-resilient cowpea, an essential crop for food security
| Autor: | Ibrahim Atokple, Yong Q. Gu, Ndiaga Cisse, Francis Kusi, Hamid Mirebrahim, Cindy Lawley, Hind Alhakami, Bao-Lam Huynh, Ming-Cheng Luo, Michael P. Timko, María Muñoz-Amatriaín, Matthew Alpert, Pei Xu, Andrew Farmer, Ousmane Boukar, Mitchell R. Lucas, Scott A. Jackson, Timothy J. Close, Christian Fatokun, Yi-Ning Guo, Serdar Bozdag, Frank M. You, Jiajie Wu, Yaqin Ma, Stefano Lonardi, Issa Drabo, Steve Wanamaker, Jeffrey D. Ehlers, Benoit Joseph Batieno, Philip A. Roberts |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
2. Zero hunger
0106 biological sciences Germplasm 0303 health sciences Bacterial artificial chromosome Genetic diversity Food security business.industry food and beverages Genomics 15. Life on land Biology 01 natural sciences Genome Biotechnology Crop 03 medical and health sciences Agriculture business 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/059261 |
| Popis: | SUMMARYCowpea (Vigna unguiculata L. Walp.) is a legume crop that is resilient to hot and drought-prone climates, and a primary source of protein in sub-Saharan Africa and other parts of the developing world. However, genome resources for cowpea have lagged behind most other major crop plants. Here we describe foundational genome resources and their application to analysis of germplasm currently in use in West African breeding programs. Resources developed from the African cultivar IT97K-499-35 include bacterial artificial chromosome (BAC) libraries and a BAC-based physical map, assembled sequences from 4,355 BACs, as well as a whole-genome shotgun (WGS) assembly. These resources and WGS sequences of an additional 36 diverse cowpea accessions supported the development of a genotyping assay for over 50,000 SNPs, which was then applied to five biparental RIL populations to produce a consensus genetic map containing 37,372 SNPs. This genetic map enabled the anchoring of 100 Mb of WGS and 420 Mb of BAC sequences, an exploration of genetic diversity along each linkage group, and clarification of macrosynteny between cowpea and common bean. The genomes of West African breeding lines and landraces have regions of marked depletion of diversity, some of which coincide with QTL that may be the result of artificial selection or environmental adaptation. The new publicly available resources and knowledge help to define goals and accelerate the breeding of improved varieties to address food security issues related to limited-input small-holder farming and climate stress. |
| Databáze: | OpenAIRE |
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