The metabolic fate of 14C-labeled immunoadjuvant peptidoglycan monomerII. In vitro studies
| Autor: | Jelka Tomašić, Ivo Hršak, Branko Ladešić, Sergije Kveder |
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| Rok vydání: | 1981 |
| Předmět: |
Biophysics
Disaccharide Peptidoglycan Kidney Biochemistry Pentapeptide repeat Amidase Mice chemistry.chemical_compound Residue (chemistry) Adjuvants Immunologic Species Specificity Animals Brevibacterium Humans Peptide bond Peptidoglycan monomer muramyl dipeptide N-acetylmuramyl-L-alanine amidase Molecular Biology Alanine Amidohydrolase fungi Glycopeptides N-Acetylmuramoyl-L-alanine Amidase carbohydrates (lipids) Blood Liver chemistry Mice Inbred CBA Acetylmuramyl-Alanyl-Isoglutamine Spleen |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 678:12-17 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(81)90042-8 |
| Popis: | Peptidoglycan monomer (GlcNAc-MurNac- L -Ala- D -isoglutamine-meso-diaminopimelic acid- D -Ala- D -Ala), labeled with 14C both in the disaccharide and pentapeptide portions, was incubated with slices of mouse liver, kidney or spleen as well as with mouse and human blood cells, plasma and serum. Peptidoglycan monomer was isolated unchanged after incubations with mouse organs and blood cells. However, upon incubation with mouse or human blood, 10–50% of the peptidoglycan monomer underwent hydrolysis to the corresponding disaccharide and pentapeptide. After incubations with plasma and serum more than 90% of the [14C]peptidoglycan monomer was metabolized: about 50% of the administered radioactive dose was recovered in the disaccharide unit and about 35% in the pentapeptide part. These results suggest that in blood, plasma and serum of mouse and man, an N- acetylmuramoyl- L -alanine amidase (mucopeptide amidohydrolase, EC 3.5.1.28) exists which splits the amide bond between the lactyl carboxyl group of the muramyl residue and the amino group of the peptide moiety in the peptidoglycan molecule. |
| Databáze: | OpenAIRE |
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