In vitro Protein Synthesis and Measurement of the Stability of Messenger RNA in the Blue-green Alga, Anabaena variabilis
| Autor: | N. G. Carr, C. K. Leach |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Peptide Biosynthesis
chemistry.chemical_classification Messenger RNA Five-prime cap Cell-Free System biology RNA Cyanobacteria biology.organism_classification Microbiology Ribosome Amino acid chemistry.chemical_compound Biochemistry chemistry Protein Biosynthesis Peptide synthesis Protein biosynthesis Aminoacylation Carbon Radioisotopes RNA Messenger Amino Acids Half-Life Anabaena variabilis |
| Zdroj: | Microbiology. 81:47-58 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-81-1-47 |
| Popis: | A cell-free preparation of the blue-green alga, Anabaena variabilis, that incorporates 14C-labelled amino acids into protein has been prepared and characterized. The activation of amino acids to amino acid transfer RNAs was characterized, and assembly of these into peptides has been described with respect to cofactor requirements and antibiotic sensitivity. The properties of these systems and the effect of antibiotics on them are similar to those of bacteria. Both natural and synthetic messenger RNA were effective in peptide formation. The kinetics of incorporation of [14C]uracil into RNA has been examined and the stability of labelled RNA from A. variabilis measured by radioactivity loss and by its role in directing peptide synthesis. The half-life of messenger RNA from this organism is approximately 12 min, and as such is comparable to that of bacteria when based upon the mean generation time of the respective organisms. |
| Databáze: | OpenAIRE |
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