| Autor: |
Zhuonan Shi, Zhe Xu, Jin Hu, Weiwei Wei, Xierong Zeng, Wei-Wei Zhao, Peng Lin |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Biosensorsbioelectronics. 201 |
| ISSN: |
1873-4235 |
| Popis: |
Heart-type fatty acid binding protein (H-FABP) has been regarded as a promising biomarker for early diagnosis of acute myocardial infarction (AMI). Developing fast and reliable method for H-FABP detection is still highly desirable but challenging. Herein, an ascorbic acid (AA)-mediated organic photoelectrochemical transistor (OPECT) sensing strategy was reported for the detection of H-FABP in phosphate buffer saline (PBS) solution and human serum. A primary antibody/H-FABP/secondary antibody-Au NPs-alkaline phosphatase (ALP) sandwich immunorecognition structure was constructed. The modified ALP could catalytically convert ascorbic acid-2-phosphate to AA, which was then analyzed by OPECT. As a result, the AA-mediated OPECT sensing strategy realized highly sensitive detection of H-FABP with a detection limit of 3.23 × 10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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