Type III Secretion Contributes to the Pathogenesis of the Soft-Rot Pathogen Erwinia carotovora: Partial Characterization of the hrp Gene Cluster
| Autor: | Anssi Rantakari, Suvi Taira, H. T. Saarilahti, S. Vähämiko, O. Virtaharju, Martin Romantschuk, E. T. Palva |
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| Rok vydání: | 2001 |
| Předmět: |
Physiology
Arabidopsis Virulence Sigma Factor Erwinia Microbiology 03 medical and health sciences Gene cluster Pseudomonas syringae Pectinase Promoter Regions Genetic Pathogen Plant Diseases 030304 developmental biology Pectin lyase 2. Zero hunger 0303 health sciences biology 030306 microbiology food and beverages General Medicine biology.organism_classification Pectobacterium carotovorum Genes Bacterial Multigene Family Agronomy and Crop Science Carbon-Oxygen Lyases |
| Zdroj: | Molecular Plant-Microbe Interactions®. 14:962-968 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi.2001.14.8.962 |
| Popis: | The virulence of soft-rot Erwinia species is dependent mainly upon secreted enzymes such as pectinases, pectin lyases, and proteases that cause maceration of plant tissue. Some soft-rot Erwinia spp. also harbor genes homologous to the hypersensitive reaction and pathogenesis (hrp) gene cluster, encoding components of the type III secretion system. The hrp genes are essential virulence determinants for numerous nonmacerating gram-negative plant pathogens but their role in the virulence of soft-rot Erwinia spp. is not clear. We isolated and characterized 11 hrp genes of Erwinia carotovora subsp. carotovora. Three putative σL-dependent Hrp box promoter sequences were found. The genes were expressed when the bacteria were grown in Hrp-inducing medium. The operon structure of the hrp genes was determined by mRNA hybridization, and the results were in accordance with the location of the Hrp boxes. An E. carotovora strain with mutated hrcC, an essential hrp gene, was constructed. The hrcC¯ strain was able to multiply and cause disease in Arabidopsis, but the population kinetics were altered so that growth was delayed during the early stages of infection. |
| Databáze: | OpenAIRE |
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