Genomic characterization of Canine circovirus detected in a dog with intermittent hemorrhagic gastroenteritis in Brazil
| Autor: | Luiz M. F. Portela, Amanda Mahnke Baccarin, João Pessoa Araújo Júnior, Thiago Neves Batista, Taís F. Cruz, Jéssica Jehle Gradiz, Ester Mariane Vieira |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Regional de Blumenau (FURB) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Circovirus
Future studies 040301 veterinary sciences Agriculture (General) Canine circovirus S1-972 0403 veterinary science 03 medical and health sciences circovirus canine circovirus medicine 030304 developmental biology 0303 health sciences General Veterinary Molecular epidemiology biology Sequencia Hemorrhagic gastroenteritis Agriculture 04 agricultural and veterinary sciences genomic characterization biology.organism_classification medicine.disease Clinical disease Molecular biology Gastroenteritis Amino acid change Genomic characterization Animal Science and Zoology Agronomy and Crop Science gastroenteritis |
| Zdroj: | Ciência Rural, Vol 50, Iss 5 (2020) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Ciência Rural v.50 n.5 2020 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
| ISSN: | 1678-4596 |
| Popis: | EnglishBecause Canine circovirus (CanineCV) is a new species of the genus Circovirus, several issues related to its epidemiology, pathogenesis and clinical disease remain unknown. Thus, this study aimed to perform the characterization of the first complete genome sequence of CanineCV detected in a dog with diarrhea in Brazil. A stool sample was collected of a ten-month-old female German Shepherd dog which had signs of intermittent hemorrhagic gastroenteritis, vomiting, and a history of eating raw pork. The complete CanineCV genome was sequenced by Next-Generation Sequencing. The sequence had 2,063 nucleotides, showed a typical genomic organization for circovirus, and was grouped with strain 214 described in the United States by phylogenetic analysis. One amino acid change was found in the replicase protein, and because of that it was considered unique to CanineCV. Therefore, the characterization of the complete genome of Brazilian CanineCV can be used in future studies of molecular epidemiology, pathogenesis and development of diagnostic tools for the prevention and control of this disease. portuguesComo o Canine circovirus (CanineCV) e uma nova especie do Genero Circovirus, varias questoes relacionadas com a sua epidemiologia, patogenia e doenca clinica permanecem desconhecidas. Assim, este estudo objetivou realizar a caracterizacao da primeira sequencia do genoma completo do CanineCV detectado em um cao com diarreia, no Brasil. Uma amostra de fezes foi coletada de um cao da raca Pastor Alemao, femea, 10 meses de idade, o qual tinha sinais de gastroenterite hemorragica intermitente, vomito e uma historia de ingestao de carne crua de porco. O genoma completo do CanineCV foi sequenciado pelo Sequenciamento de Nova Geracao. A sequencia tinha 2.063 nucleotideos, apresentou uma organizacao genomica tipica para um circovirus e foi agrupado com a cepa 214, descrita nos Estados Unidos pela analise filogenetica. Uma mudanca de aminoacido foi encontrada na proteina de replicacao e por causa disso ela foi considerada unica para o CanineCV. Portanto, a caracterizacao do genoma completo do CanineCV brasileiro pode ser utilizada em futuros estudos de epidemiologia molecular, patogenia e no desenvolvimento de ferramentas de diagnostico para prevencao e controle desta doenca. |
| Databáze: | OpenAIRE |
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