Separation of diploid from aneuploid cells in acute lymphoblastic leukaemia
| Autor: | A. J. Walle, W. Niedermayer |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Pathology
medicine.medical_specialty Blood Cells medicine.diagnostic_test Separation (statistics) Complete remission Cell Separation DNA Hematology Biology Aneuploidy Diploidy Leukemia Lymphoid Flow cytometry medicine.anatomical_structure Cellular dna Centrifugation Density Gradient medicine Cancer research Humans Lymphoblastic leukaemia Aneuploid Cells Bone marrow Ploidy |
| Zdroj: | British Journal of Haematology. 57:571-576 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1984.tb02934.x |
| Popis: | Summary. A new method for the separation of diploid and aneuploid cells from blood of patients with leukaemia is described. Density gradient separation using a modified silica particle gradient was combined with analysis of cellular DNA content by flow cytometry. Submicroscopic levels of diploid or aneuploid cells were enriched up to 90% purity. Three patients with acute lymphoblastic leukaemia and no evidence of aneuploid cells after separation are in continuing complete remission after 24.5–47 months. Two patients with aneuploid cells detected after separation expired in relapse 0.5 and 3 months later. Cell separation prior to flow cytometric analysis might be a useful tool to detect submicroscopic levels of aneuploid leukaemic cells in blood and bone marrow, and to predict impending relapse. |
| Databáze: | OpenAIRE |
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