Alternative splicing of the eag potassium channel gene in Drosophila generates a novel signal transduction scaffolding protein
| Autor: | Leslie C. Griffith, Xiu Xia Sun, S. Lynn Bostrom |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Scaffold protein
Cell signaling Recombinant Fusion Proteins Context (language use) Biology Article Cellular and Molecular Neuroscience Chlorocebus aethiops Animals Drosophila Proteins Molecular Biology Protein Kinase C Alternative splicing Cell Biology Cyclic AMP-Dependent Protein Kinases Ether-A-Go-Go Potassium Channels Potassium channel Associative learning Cell biology Enzyme Activation Alternative Splicing Drosophila melanogaster Biochemistry COS Cells Signal transduction Nuclear localization sequence Signal Transduction |
| Zdroj: | Molecular and Cellular Neuroscience. 40:338-343 |
| ISSN: | 1044-7431 |
| Popis: | The Drosophila eag gene has been shown to regulate neuronal excitability (Wu et al., 1983), olfaction (Dubin et al., 1998), associative learning (Griffith et al., 1994) and larval locomotion (Wang et al., 2002a). Not all of the roles of this gene in these processes can be explained by its function as a voltage-gated potassium channel (e.g. Zhong and Wu, 1991). In this study, we show that the eag gene is spliced in a PKA- and PKC-regulated manner to produce a protein lacking channel domains. This protein, in the context of activated PKA, can engage cellular signaling pathways that alter cell structure. Nuclear localization is necessary for C-terminal-mediated effects, which also require MAPK. The requirement for PKA/PKC activation in the synthesis and function of this novel protein suggests that it may couple membrane events to nuclear signaling to regulate neuronal function on long time scales. |
| Databáze: | OpenAIRE |
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