Effects of Estradiol on Autophagy and Nrf-2/ARE Signals after Cerebral Ischemia
| Autor: | Xiaoli Dong, Sujuan Sun, Litao Li, Jing-Hong Chen, Jian-Hua Wang, Jingru Zhao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pro inflammation NF-E2-Related Factor 2 Physiology Ischemia Female sex hormones Disease Biology Response Elements medicine.disease_cause Neuroprotection lcsh:Physiology Brain Ischemia lcsh:Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Internal medicine Autophagy medicine Animals lcsh:QD415-436 Stroke lcsh:QP1-981 Estradiol Incidence (epidemiology) Cerebral ischemia medicine.disease Oxidative Stress 030104 developmental biology Endocrinology Pro-inflammation Female 030217 neurology & neurosurgery Oxidative stress Signal Transduction |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 41, Iss 5, Pp 2027-2036 (2017) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Estradiol (EST) reduces the risk of stroke and decreases the incidence and progression of the disease because of its neuroprotective roles in inhibiting cell death that occurs in response to a variety of neuronal stimuli such as inflammation and oxidative stress. In this study, we determined the role played by autophagy and Nrf2-ARE signal pathways in the hippocampus regions in modulating cerebral ischemia under different EST conditions. Methods: Western blot analysis and ELISA were used to determine the protein expression of autophagy and Nrf2-ARE pathways; and the levels of pro-inflammatory cytokines (PICs) and a key marker of oxidative stress. Results: Lacking of EST amplifies autophagy and attenuates Nrf2-ARE pathway in the hippocampus CA1 region. Blocking autophagy alleviates neurological deficits following cerebral ischemia with lacking of EST levels and the effects of autophagy are associated with PIC and oxidative stress. Conclusions: EST influences the protein expression of autophagy and Nrf2-ARE signaling in the brain, which is linked to the pathophysiological processes of PICs and oxidative stress. Moreover, inhibition of autophagy plays a beneficial role in modulating neurological deficits after cerebral ischemia observed under conditions of a lower level of EST. |
| Databáze: | OpenAIRE |
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