Two-dimensional electrophoresis and characterization of antigens from Paracoccidioides brasiliensis
| Autor: | W.A. Brito, M. Sueli S. Felipe, Célia Maria de Almeida Soares, Rosália Santos Amorim Jesuino, Cristiane Amorim Fonseca, Daniela A. Cunha |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Antigens
Fungal Immunology Immunoblotting Molecular Sequence Data Biology Microbiology Malate dehydrogenase Triosephosphate isomerase Fungal Proteins Antigen medicine Humans Electrophoresis Gel Two-Dimensional Amino Acid Sequence chemistry.chemical_classification Paracoccidioides brasiliensis Sequence Homology Amino Acid Paracoccidioidomycosis Aldolase A Paracoccidioides biology.organism_classification medicine.disease Yeast Amino acid Infectious Diseases chemistry Biochemistry Immunoglobulin G biology.protein |
| Zdroj: | Microbes and infection. 3(7) |
| ISSN: | 1286-4579 |
| Popis: | Paracoccidioides brasiliensis is a fungal pathogen of humans. To identify antigens from P. brasiliensis we fractionated a crude preparation of proteins from the fungus and detected the IgG reactive proteins by immunoblot assays of yeast cellular extracts with sera of patients with paracoccidioidomycosis (PCM). We identified and characterized six new antigens by amino acid sequencing and homology search analyses with other proteins deposited in a database. The newly characterized antigens were highly homologous to catalase, fructose-1,6-biphosphate aldolase (aldolase), glyceraldehyde-3-phosphate dehydrogenase, malate dehydrogenase and triosephosphate isomerase from several sources. The characterized antigens presented preferential synthesis in yeast cells, the host fungus phase. |
| Databáze: | OpenAIRE |
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