Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing
| Autor: | Lirong Zhang, Yang Liu, Hui Chen, Mingqin Shao, Chunxin Li, Xiangyang Xu, Guihua Bai, Yuhui Pang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agriculture (General) KASP Single-nucleotide polymorphism Plant Science 01 natural sciences Rust S1-972 03 medical and health sciences Wheat leaf rust Diagnostic marker Aegilops tauschii Lr42 Allele Gene Genetics biology Haplotype food and beverages Agriculture biology.organism_classification 030104 developmental biology Genetic marker Triticum tauschii Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Crop Journal, Vol 9, Iss 6, Pp 1357-1366 (2021) |
| ISSN: | 2214-5141 |
| Popis: | Wheat leaf rust is a prevalent foliar disease in wheat worldwide. Growing resistant cultivars is an effective strategy to minimize the impact of leaf rust on yield and grain quality. Lr42 is a leaf rust resistance gene identified from Aegilops tauschii and is still effective against current predominant leaf rust races in the United States and many other countries. In this study, we developed diagnostic DNA markers for Lr42 using the sequence polymorphisms of a differentially expressed gene (TaRPM1) encoding a putative NB-ARC protein in the Lr42 candidate region identified by RNA-sequencing of two near-isogenic lines contrasting in Lr42 alleles. Markers were designed based on a deletion mutation and a single nucleotide polymorphism (SNP) in the gene. Haplotype analyses of the newly developed markers in the three diversity panels demonstrated that they are diagnostic for Lr42, and superior to previously used markers in selection accuracy. These markers have the advantages of low cost and easy assay, and they are suitable for marker-assisted selection in breeding programs with either high- or low-throughput marker screening facilities. |
| Databáze: | OpenAIRE |
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