Rupture of Lipid Membranes Induced by Amphiphilic Janus Nanoparticles
| Autor: | Xianqiao Wang, Liuyang Zhang, Yan Yu, Yi Yi, Kwahun Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Surface Properties
Chemistry Lipid Bilayers General Engineering General Physics and Astronomy Nanoparticle Janus particles 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Surface-Active Agents Surface coating Membrane Chemical engineering Amphiphile Nanoparticles Particle General Materials Science Janus Particle Size 0210 nano-technology Lipid bilayer |
| Zdroj: | ACS Nano. 12:3646-3657 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.8b00759 |
| Popis: | The surface coatings of nanoparticles determine their interaction with biomembranes, but studies have been limited almost exclusively to nanoparticles with a uniform surface chemistry. Although nanoparticles are increasingly made with complex surface chemistries to achieve multifunctionalities, our understanding of how a heterogeneous surface coating affects particle-biomembrane interaction has been lagging far behind. Here we report an investigation of this question in an experimental system consisting of amphiphilic "two-faced" Janus nanoparticles and supported lipid membranes. We show that amphiphilic Janus nanoparticles at picomolar concentrations induce defects in zwitterionic lipid bilayers. In addition to revealing the various effects of hydrophobicity and charge in particle-bilayer interactions, we demonstrate that the Janus geometry-the spatial segregation of hydrophobicity and charges on particle surface-causes nanoparticles to bind more strongly to bilayers and induce defects more effectively than particles with uniformly mixed surface functionalities. We combine experiments with computational simulation to further elucidate how amphiphilic Janus nanoparticles extract lipids to rupture intact lipid bilayers. This study provides direct evidence that the spatial arrangement of surface functionalities on a nanoparticle, rather than just its overall surface chemistry, plays a crucial role in determining how it interacts with biological membranes. |
| Databáze: | OpenAIRE |
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