Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium
| Autor: | V. La Bella, R. Guarneri, S Scondotto, Giovanni Savettieri, Giuseppe Salemi, Federico Piccoli, Donatella Ferraro |
|---|---|
| Přispěvatelé: | Savettieri, G., Guarneri, R., Salemi, G., LA BELLA, V., Ferraro, D., Scondotto, S., Piccoli, F. |
| Rok vydání: | 1990 |
| Předmět: |
Central nervous system
Flunitrazepam Biology Settore BIO/19 - Microbiologia Generale Biochemistry gamma-Aminobutyric acid GABA Cellular and Molecular Neuroscience medicine Animals Binding site Cells Cultured gamma-Aminobutyric Acid Neurons Fetus Cell Differentiation General Medicine Hormones In vitro Culture Media Cell biology medicine.anatomical_structure neuronal culture Cell culture Cerebral cortex Settore MED/26 - Neurologia Neuroscience medicine.drug |
| Zdroj: | Neurochemical Research. 15:773-776 |
| ISSN: | 1573-6903 0364-3190 |
| Popis: | [3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink