Modulation of IL-1 β gene expression by lipid peroxidation inhibition after kainic acid-induced rat brain injury
| Autor: | Herbert Marini, Floriana Laureanti, Maria Passaniti, Maria Bellomo, Francesco Squadrito, Domenica Altavilla, Elena Bianca Adamo, Letteria Minutoli, Alessandra Bitto, Paolo Seminara, Rolando Marini, Maria Carmela Bonaccorso, Gioacchino Calapai |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Kainic acid medicine.medical_specialty Neurotoxins Central nervous system Brain Edema Brain damage Biology Hippocampus Rats Sprague-Dawley Lipid peroxidation chemistry.chemical_compound Developmental Neuroscience Seizures Malondialdehyde Internal medicine Convulsion medicine Animals Hippocampus (mythology) RNA Messenger Benzofurans Cerebral Cortex Kainic Acid Behavior Animal Glutathione Rats Disease Models Animal Oxidative Stress medicine.anatomical_structure Endocrinology Gene Expression Regulation Neurology chemistry Biochemistry Brain Injuries Nerve Degeneration Lipid Peroxidation medicine.symptom Interleukin-1 |
| Zdroj: | Experimental Neurology. 188:178-186 |
| ISSN: | 0014-4886 |
| Popis: | Brain injury was induced by intraperitoneal administration of kainic acid (KA, 10 mg/kg). Animals were randomized to receive either IRFI 042 (20 mg/kg i.p.), a lipid peroxidation inhibitor, or its vehicle (NaCl 0.9% DMSO 10% 1 ml/kg i.p.) 30 min before KA administration. A first set of animals was sacrificed 6 h after KA injection to measure malondialdehyde (MDA) content, glutathione-reduced (GSH) levels and the mRNA for interleukin-1beta (IL-1beta) in the cortex and in the hippocampus. A second set of animals was sacrificed 48 h after KA administration for histological analysis. All animals were observed for monitoring the behavioral sequelae and for evaluating latency of convulsions. Sham brain injury rats were used as controls. Intraperitoneal administration of IRFI 042 significantly decreased brain MDA (cortex: KA + vehicle = 0.285 +/- 0.04 nmol/mg protein; KA + IRFI 042 = 0.156 +/- 0.02 nmol/mg protein, P0.005; hippocampus: KA + vehicle = 0.350 +/- 0.03 nmol/mg protein; KA + IRFI 042 = 0.17 +/- 0.04 nmol/mg protein, P0.005), prevented the brain loss of GSH in both cortex (KA + vehicle = 7.81 +/- 1 micromol/g protein; KA + IRFI 042 = 12.1 +/- 1 micromol/g protein; P0.005) and hippocampus (KA + vehicle = 5 +/- 0.8 micromol/g protein; KA + IRFI 042 = 9.4 +/- 1.8 micromol/g protein; P0.005), reduced both brain IL-1beta mRNA expression and oedema, and increased latency of convulsions. Histological analysis showed a reduction of cell damage in IRFI 042-treated samples. The present data indicate that lipid peroxidation inhibition reduces IL-1beta gene expression and protects against kainic acid-induced brain damage. |
| Databáze: | OpenAIRE |
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