Inhibition of Bacterial Ice Nucleators Is Not an Intrinsic Property of Antifreeze Proteins
| Autor: | Ralph Schwidetzky, Hans Ramløv, Arthur L. DeVries, Mischa Bonn, Konrad Meister, Anna T. Kunert, Janine Fröhlich-Nowoisky, Ulrich Pöschl |
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| Rok vydání: | 2020 |
| Předmět: |
Bacteria
010304 chemical physics Chemistry Ice digestive oral and skin physiology 010402 general chemistry 01 natural sciences digestive system diseases 0104 chemical sciences Surfaces Coatings and Films Bacterial protein Bacterial Proteins Biochemistry Antifreeze protein Antifreeze Proteins Freezing embryonic structures 0103 physical sciences Materials Chemistry Ice nucleus Physical and Theoretical Chemistry neoplasms |
| Zdroj: | The Journal of Physical Chemistry B. 124:4889-4895 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/acs.jpcb.0c03001 |
| Popis: | Cold-adapted organisms use antifreeze proteins (AFPs) or ice-nucleating proteins (INPs) for the survival in freezing habitats. AFPs have been reported to be able to inhibit the activity of INPs, a property that would be of great physiological relevance. The generality of this effect is not understood, and for the few known examples of INP inhibition by AFPs, the molecular mechanisms remain unclear. Here, we report a comprehensive evaluation of the effects of five different AFPs on the activity of bacterial ice nucleators using a high-throughput ice nucleation assay. We find that bacterial INPs are inhibited by certain AFPs, while others show no effect. Thus, the ability to inhibit the activity of INPs is not an intrinsic property of AFPs, and the interactions of INPs and different AFPs proceed through protein-specific rather than universal molecular mechanisms. |
| Databáze: | OpenAIRE |
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