Proteomic characterization of low molecular weight allergens and putative allergen proteins in lentil (Lens culinaris) cultivars of Bangladesh
| Autor: | Oumma Halima, Nagib Ahsan, Nazma Shaheen, R. Shyama Prasad Rao, Kazi Turjaun Akhter, Noshin Nuzhat, Rashaun S. Wilson |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Biology medicine.disease_cause 01 natural sciences Analytical Chemistry 0404 agricultural biotechnology Allergen Tandem Mass Spectrometry medicine Cultivar Amino Acid Sequence Peptide sequence Chromatography High Pressure Liquid Plant Proteins Bangladesh 010401 analytical chemistry Selected reaction monitoring 04 agricultural and veterinary sciences General Medicine Allergens 040401 food science 0104 chemical sciences Molecular Weight Biochemistry Proteome Lens Plant Quantitative analysis (chemistry) Plant lipid transfer proteins Sequence Alignment Food Science |
| Zdroj: | Food chemistry. 297 |
| ISSN: | 1873-7072 |
| Popis: | Here, we present the proteome profiling of low-molecular weight (50 kDa) proteins of seven different lentil cultivars developed by Bangladesh Agricultural Research Institute. A total of 2873 peptides corresponding to 180 unique proteins were identified wherein24% of them were described lentil allergens. Comparative relative quantitation showed differences in protein abundance of major allergen proteins such as Len c 1.0101, Len c 1.0102, and lipid transfer proteins (LTPs), indicating qualitative and quantitative variations in allergen proteins in lentil cultivars. In this report, for the first-time, the amino acid sequence of LTPs in lentil has been confirmed by high resolution mass spectrometry. In addition, ideal peptides of Len c 1.0101, Len c 1.0102, and LTPs allergens were further determined with multiple reaction monitoring (MRM) analysis. Therefore, this data could provide a great resource for further development of targeted, proteomics-based assays for quantification of lentil allergens. |
| Databáze: | OpenAIRE |
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