Outer membrane vesicles mediated horizontal transfer of an aerobic denitrification gene between Escherichia coli
| Autor: | Yang Luo, Lianjie Wang, Weichuan Qiao, Jiahui Miao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Bioengineering 010501 environmental sciences medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences Plasmid Aerobic denitrification Escherichia coli medicine Environmental Chemistry 0105 earth and related environmental sciences 0303 health sciences biology 030306 microbiology Chemistry technology industry and agriculture equipment and supplies biology.organism_classification Nitrite reductase Pollution Pseudomonas stutzeri Transformation (genetics) Biodegradation Environmental Denitrification Bacterial outer membrane Bacteria Plasmids |
| Zdroj: | Biodegradation. 32:435-448 |
| ISSN: | 1572-9729 0923-9820 |
| DOI: | 10.1007/s10532-021-09945-y |
| Popis: | Bacterial genetic material can be horizontally transferred between microorganisms via outer membrane vesicles (OMVs) released by bacteria. Up to now, the application of vesicle-mediated horizontal transfer of “degrading genes” in environmental remediation has not been reported. In this study, the nirS gene from an aerobic denitrification bacterium, Pseudomonas stutzeri, was enclosed in a pET28a plasmid, transformed into Escherichia coli (E. coli) DH5α and expressed in E. coli BL21. The E. coli DH5α released OMVs containing the recombination plasmid pET28a–nirS-EGFP. When compared with the free pET28a–nirS-EGFP plasmid’s inability to transform, nirS in OMVs could be transferred into E. coli BL21 with the transformation frequency of 2.76 × 106 CFU/g when the dosage of OMVs was 200 µg under natural conditions, and nirS could express successfully in recipient bacteria. Furthermore, the recipient bacteria that received OMVs containing pET28a–nirS-EGFP could produce 18.16 U/mL activity of nitrite reductase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink