Confined Diffusion Without Fences of a G-Protein-Coupled Receptor as Revealed by Single Particle Tracking
| Autor: | Nicolas Destainville, Claire Millot, Laurence Salomé, David S. Dean, André Lopez, Frédéric Daumas |
|---|---|
| Přispěvatelé: | Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physique Statistique des Systèmes Complexes (LPT) (PhyStat), Laboratoire de Physique Théorique (LPT), Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) |
| Jazyk: | angličtina |
| Předmět: |
MESH: Signal Transduction
Receptors Opioid mu Analytical chemistry Gold Colloid Kidney Diffusion Cell membrane 0302 clinical medicine Bacteriophage T7 Nanotechnology [PHYS.COND.CM-SM]Physics [physics]/Condensed Matter [cond-mat]/Statistical Mechanics [cond-mat.stat-mech] Diffusion (business) MESH: Nanotechnology MESH: Receptors Cell Surface 0303 health sciences Microscopy Video Chemistry MESH: Models Chemical MESH: Diffusion Microspheres MESH: Staining and Labeling medicine.anatomical_structure Membrane Colloidal gold Signal transduction Signal Transduction MESH: GTP-Binding Proteins MESH: Motion MESH: Microspheres Biophysics Receptors Cell Surface MESH: Receptors Opioid mu Models Biological Cell Line Motion 03 medical and health sciences GTP-Binding Protein Regulators GTP-Binding Proteins MESH: GTP-Binding Protein Regulators medicine [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Particle Size Particle Size MESH: Gold Colloid 030304 developmental biology G protein-coupled receptor Membranes Staining and Labeling Cell Membrane MESH: Bacteriophage T7 MESH: Models Biological MESH: Kidney Fibroblasts MESH: Cell Line MESH: Microscopy Video Models Chemical Membrane protein MESH: Fibroblasts Particle size 030217 neurology & neurosurgery MESH: Cell Membrane |
| Zdroj: | Biophysical Journal Biophysical Journal, Biophysical Society, 2003, 84 (1), pp.356-66 |
| ISSN: | 0006-3495 1542-0086 |
| DOI: | 10.1016/S0006-3495(03)74856-5 |
| Popis: | International audience; Single particle tracking is a powerful tool for probing the organization and dynamics of the plasma membrane constituents. We used this technique to study the micro -opioid receptor belonging to the large family of the G-protein-coupled receptors involved with other partners in a signal transduction pathway. The specific labeling of the receptor coupled to a T7-tag at its N-terminus, stably expressed in fibroblastic cells, was achieved by colloidal gold coupled to a monoclonal anti T7-tag antibody. The lateral movements of the particles were followed by nanovideomicroscopy at 40 ms time resolution during 2 min with a spatial precision of 15 nm. The receptors were found to have either a slow or directed diffusion mode (10%) or a walking confined diffusion mode (90%) composed of a long-term random diffusion and a short-term confined diffusion, and corresponding to a diffusion confined within a domain that itself diffuses. The results indicate that the confinement is due to an effective harmonic potential generated by long-range attraction between the membrane proteins. A simple model for interacting membrane proteins diffusion is proposed that explains the variations with the domain size of the short-term and long-term diffusion coefficients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|