RAD54 promotes alternative lengthening of telomeres by mediating branch migration
| Autor: | Ying Jie Lock, Rachel Litman Flynn, Katherine Terranova, Lisa M Carson, Nicholas Lupo, Emily Mason-Osann |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
DNA Replication
DNA Repair Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Report Genetics Strand invasion alternative lengthening of telomeres Molecular Biology Mitosis DNA Polymerase III 030304 developmental biology Anaphase 0303 health sciences DNA synthesis fungi DNA Replication Repair & Recombination Telomere Homeostasis Telomere telomeres Branch migration Homologous Recombination Pathway Cell biology chemistry break‐induced replication RAD54 030217 neurology & neurosurgery DNA Reports |
| Zdroj: | EMBO Reports |
| ISSN: | 1469-3178 1469-221X |
| DOI: | 10.15252/embr.201949495 |
| Popis: | Cancer cells can activate the alternative lengthening of telomeres (ALT) pathway to promote replicative immortality. The ALT pathway promotes telomere elongation through a homologous recombination pathway known as break‐induced replication (BIR), which is often engaged to repair single‐ended double‐stranded breaks (DSBs). Single‐ended DSBs are resected to promote strand invasion and facilitate the formation of a local displacement loop (D‐loop), which can trigger DNA synthesis, and ultimately promote telomere elongation. However, the exact proteins involved in the maturation, migration, and resolution of D‐loops at ALT telomeres are unclear. In vitro, the DNA translocase RAD54 both binds D‐loops and promotes branch migration suggesting that RAD54 may function to promote ALT activity. Here, we demonstrate that RAD54 is enriched at ALT telomeres and promotes telomeric DNA synthesis through its ATPase‐dependent branch migration activity. Loss of RAD54 leads to the formation of unresolved recombination intermediates at telomeres that form ultra‐fine anaphase bridges in mitosis. These data demonstrate an important role for RAD54 in promoting ALT‐mediated telomere synthesis. The DNA repair and recombination protein RAD54 is enriched at ALT telomeres. RAD54 promotes DNA synthesis at ALT telomeres through its ATPase and branch migration activities. |
| Databáze: | OpenAIRE |
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