MUC Expression in Gallbladder Epithelial Tissues in Cholesterol-Associated Gallbladder Disease
| Autor: | Ho Soon Choi, Byung Chul Yoon, Oh Young Lee, Dae Won Jun, Seung Sam Paik, Yong Seok Kim, Kyeong Geun Lee, Hang Lak Lee, Jin Lee, Kyo Sang Yoo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Hypercholesterolemia Gallbladder disease Gallstones Gallbladder Diseases Mucin 5AC MUC5B Pathogenesis 03 medical and health sciences chemistry.chemical_compound Dogs 0302 clinical medicine Cholecystitis medicine Animals Humans Mucin-6 Mucin-3 Hepatology business.industry Cholesterol Gastric Mucins Gallbladder Mucin Gastroenterology Case-control study Epithelial Cells medicine.disease Mucin-5B 030104 developmental biology medicine.anatomical_structure chemistry Case-Control Studies Immunology MUC3 Original Article 030211 gastroenterology & hepatology Infection business |
| Zdroj: | Gut and Liver |
| ISSN: | 2005-1212 1976-2283 |
| DOI: | 10.5009/gnl15600 |
| Popis: | Background/Aims Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterol-associated gallbladder disease and evaluated the relationship between mucin and bacterial infection. Methods The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. Results MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. Conclusions The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression. |
| Databáze: | OpenAIRE |
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