Influence of ACTH on phosphorylation of ribosomal proteins from rat adrenal glands
| Autor: | N. A. Yudaev, M. A. Krekhova, M. K. Chekhranova |
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| Rok vydání: | 1980 |
| Předmět: |
Male
Ribosomal Proteins Adrenocorticotropic hormone Biology In Vitro Techniques Phosphorylation Process chemistry.chemical_compound Adrenocorticotropic Hormone Ribosomal protein Corticosterone Adrenal Glands medicine Cyclic AMP Animals Phosphorylation Polyacrylamide gel electrophoresis Adrenal cortex Adrenal gland General Neuroscience Electrophoresis Disc Molecular biology Rats medicine.anatomical_structure chemistry Biochemistry Protein Kinases |
| Zdroj: | Neuroscience and behavioral physiology. 10(1) |
| ISSN: | 0097-0549 |
| Popis: | 1. When adrenal tissue is incubated in Krebs-Ringer-bicarbonate medium containing [32P]-labeled KH2PO4, the radioactive phosphate is incorporated into the ribosomal proteins. On the average ACTH stimulates the incorporation of phosphate into the total proteins by 38%. 2. Ribosomal proteins are separated into 30 fractions by electrophoresis in polyacrylamide gel at pH 4.5, five to six of which are phosphorylated during incubation. ACTH added to the incubation medium changes the phosphorylation process: stimulation of phosphorylation is observed in two protein fractions and inhibition in three. 3. A change in the phosphorylation of the ribosomal proteins maybe one of the mechanisms through which the effect of ACTH is realized in cells of the adrenal cortex. |
| Databáze: | OpenAIRE |
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