Ultrasensitive electrochemiluminescence immunosensor with wide linear range based on a multiple amplification approach
| Autor: | Hong-Yuan Chen, Jing-Juan Xu, Nan Zhang, Xiao-Lei Huo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Chromatography biology Polyglutamic acid 02 engineering and technology Conjugated system 010402 general chemistry 021001 nanoscience & nanotechnology Platinum nanoparticles 01 natural sciences 0104 chemical sciences Luminol lcsh:Chemistry chemistry.chemical_compound chemistry Linear range lcsh:Industrial electrochemistry lcsh:QD1-999 Electrochemistry biology.protein Electrochemiluminescence Glucose oxidase 0210 nano-technology lcsh:TP250-261 |
| Zdroj: | Electrochemistry Communications, Vol 98, Iss, Pp 33-37 (2019) |
| ISSN: | 1388-2481 |
| Popis: | An ultrasensitive and highly selective electrochemiluminescence (ECL) immunosensor is proposed for the detection of prostate specific antigen (PSA) based on multiple amplification via glucose oxidase (GOx) and platinum nanoparticles (Pt NPs) conjugated with polyglutamic acid (PGA). The proposed immunosensor has a wide linear range (1.0 pg/mL–150 ng/mL) and low detection limit (0.6 pg/mL) for PSA detection, which is due in part to the excellent catalytic activity of the Ab2-GOx-PGA-Pt NPs probe toward the luminol ECL reaction. Keywords: Ultrasensitive, Electrochemiluminescence, Multiple amplification, PGA, PSA |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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