Characterization of bovine MHC DRB3 diversity in Latin American Creole cattle breeds
| Autor: | Guillermo Giovambattista, Hideki Saito, Luz Ángela Álvarez Franco, Yuki Matsumoto, Shin-nosuke Takeshima, Misao Onuma, Yoko Aida, María Verónica Ripoli |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Genetic Markers
Latin Americans Latin American Creole cattle media_common.quotation_subject Molecular Sequence Data Creole language Sequence-based typing Breeding Biology Major histocompatibility complex Polymerase Chain Reaction Yacumeño Gene Frequency Genetics Animals Amino Acid Sequence Selection Genetic Sequence-based Typing Alleles Phylogeny media_common Principal Component Analysis Polymorphism Genetic Major Histocompatibility Complex Class II Histocompatibility Antigens Class II Hartón del Valle Exons General Medicine Producción Animal y Lechería Major histocompatibility complex class II CIENCIAS AGRÍCOLAS biology.protein Ethnology Cattle BoLA-DRB3 polymorphism Diversity (politics) |
| Popis: | In cattle, bovine leukocyte antigens (BoLAs) have been extensively used as markers for diseases and immunological traits. However, none of the highly adapted Latin American Creole breeds have been characterized for BoLA gene polymorphism by high resolution typing methods. In this work, we sequenced exon 2 of the BoLA class II DRB3 gene from 179 cattle (113 Bolivian Yacumeño cattle and 66 Colombian Hartón del Valle cattle breeds) using a polymerase chain reaction sequence-based typing (PCR-SBT) method. We identified 36 previously reported alleles and three novel alleles. Thirty-five (32 reported and three new) and 24 alleles (22 reported and two new) were detected in Yacumeño and Hartón del Valle breeds, respectively. Interestingly, Latin American Creole cattle showed a high degree of gene diversity despite their small population sizes, and 10 alleles including three new alleles were found only in these two Creole breeds. We next compared the degree of genetic variability at the population and sequence levels and the genetic distance in the two breeds with those previously reported in five other breeds: Holstein, Japanese Shorthorn, Japanese Black, Jersey, and Hanwoo. Both Creole breeds presented gene diversity higher than 0.90, a nucleotide diversity higher than 0.07, and mean number of pairwise differences higher than 19, indicating that Creole cattle had similar genetic diversity at BoLA-DRB3 to the other breeds. A neutrality test showed that the high degree of genetic variability may be maintained by balancing selection. The FST index and the exact G test showed significant differences across all cattle populations (FST = 0.0478; p < 0.001). Results from the principal components analysis and the phylogenetic tree showed that Yacumeño and Hartón del Valle breeds were closely related to each other. Collectively, our results suggest that the high level of genetic diversity could be explained by the multiple origins of the Creole germplasm (European, African and Indicus), and this diversity might be maintained by balancing selection. Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Takeshima, Shin Nosuke. Riken. Viral Infectious Diseases Unit; Japón Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Matsumoto, Yuki. Riken. Viral Infectious Diseases Unit; Japón Fil: Alvarez Franco, Luz Angela. Universidad Nacional de Colombia; Colombia Fil: Saito, Hideki. Uganda Animal Disease Control Project at Makerere University; Uganda Fil: Onuma, Misao. Riken. Viral Infectious Diseases Unit; Japón Fil: Aida, Yoko. Riken. Viral Infectious Diseases Unit; Japón |
| Databáze: | OpenAIRE |
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