Effect of recombinant feline interferon-omega alone and in combination with chemotherapeutic agents on putative tumour-initiating cells and daughter cells derived from canine and feline mammary tumours
| Autor: | David J. Argyle, C. Penzo, Rhona Muirhead, Roderick Else, Martin Ross |
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| Rok vydání: | 2009 |
| Předmět: |
Cell division
Antineoplastic Agents Mammary Neoplasms Animal Biology Cat Diseases Feline Mammary Carcinoma Dogs Interferon medicine Carcinoma Animals Dog Diseases Cells Cultured Canine Mammary Carcinoma General Veterinary Dose-Response Relationship Drug medicine.disease Virology In vitro Recombinant Proteins Cell culture Interferon Type I Cancer research Cats Female Stem cell Drug Screening Assays Antitumor medicine.drug |
| Zdroj: | Veterinary and comparative oncology. 7(4) |
| ISSN: | 1476-5829 |
| Popis: | Interferons (IFNs) are naturally produced cytokines with multiple important biological functions. The activity of recombinant feline IFN-omega (rFeIFN-omega) alone and in combination with chemotherapeutic drugs was tested on canine and feline mammary carcinoma cell lines (REM134 and CAT-MT) and putative tumour-initiating cells (TIC) derived from these cell lines by sphere assay. Viability was measured by chemoluminescence and a one-way analysis of variance and Student's t-tests were used for statistical analysis. rFeIFN-omega showed in vitro antitumour activity on feline and canine mammary carcinoma cells and putative TICs with a dose-dependent and target cell-specific action. Putative TICs were more resistant to the action of rFeIFN-omega compared with daughter REM134 and CAT-MT cells. REM134 cells and TICs were more sensitive to rFeIFN-omega compared with the feline counterparts. An additive effect was noticed between rFeIFN-omega and conventional anticancer drugs, in particular following co-culture of cells with anthracycline drugs. The results suggest that rFeIFN-omega warrants further investigation as a therapeutic adjunct in feline and canine mammary tumours. |
| Databáze: | OpenAIRE |
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