Capillary fractionation of HPLC substrates by a microfluidic droplet generator for high throughput analysis
| Autor: | Shereef Hamed, Amar S. Basu, Brian J. Shay |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Chromatography
Materials science Capillary action Microfluidics Fraction (chemistry) Fractionation Chemical Fractionation Capillary number High-Throughput Screening Assays Volumetric flow rate Surface tension Reagent Silicone Oils Coloring Agents Rheology Chromatography High Pressure Liquid Oleic Acid |
| Zdroj: | EMBC |
| DOI: | 10.1109/iembs.2011.6092071 |
| Popis: | Biochemical samples are complex mixtures containing 1000's of components which often must be fractionated prior to analysis. Conventional fraction collectors, which can only accommodate 10's of fractions, are not well suited for high throughput analysis. This paper describes microfractionation in droplets (μFD), a scalable microfluidic technique for generating thousands of fractions. A drop generator, placed downstream from a high performance liquid chromatography (HPLC) column, encapsulates the separated components into a serial array of monodisperse droplets. The droplets can be stored in a capillary or immediately used in subsequent assays. Using μFD, a mixture of 3 dyes separated in a C18 column was fractionated into 2,160 droplets in |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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