Identification of low-abundance differentially expressed transcripts using arrayed cDNA clones
| Autor: | S.K Stephens, J.F Burke, P Golby, J.P Rast |
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| Rok vydání: | 2002 |
| Předmět: |
Male
DNA Complementary Transcription Genetic Physiology Biology Biochemistry Polymerase Chain Reaction Complementary DNA Gene expression Animals Drosophila Proteins RNA Messenger Molecular Biology Sequence (medicine) Gene Library Oligonucleotide Array Sequence Analysis Whole genome sequencing Genetics Expressed sequence tag cDNA library Gene Expression Profiling Nucleic Acid Hybridization Nylons Drosophila melanogaster Suppression subtractive hybridization Female DNA microarray |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 133(4) |
| ISSN: | 1096-4959 |
| Popis: | Analysis of comparative gene expression by the use of DNA microarrays has become a widely used tool. However, this technique is only readily applied to organisms where sequence information is known. This paper describes the development of a low-cost method of gene discovery by enrichment of differentially expressed transcripts, which uses cDNA library arrays of bacterial clones on nylon membranes (macroarrays) coupled with a subtractive probe preparation method to discover differentially expressed genes. The method requires no prior knowledge of the organism's genome sequence and overcomes the inherent insensitivity of standard methods of macroarray hybridisation. |
| Databáze: | OpenAIRE |
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