Enzymic assay of glycerol, dihydroxyacetone, and glyceraldehyde

Autor:	John A. Watson, James A. Hayashi, Joyce K. Pinter
Rok vydání:	1967
Předmět:	Glycerol Glycerol kinase Phosphotransferases Biophysics Dihydroxyacetone Ketones Glyceraldehyde Biochemistry chemistry.chemical_compound Sodium borohydride chemistry Spectrophotometry Reagent Yeasts Pyruvic acid Molecular Biology Oxidation-Reduction Pyruvate kinase
Zdroj:	Archives of biochemistry and biophysics. 121(2)
ISSN:	0003-9861
Popis:	An enzymic glycerol assay in which glycerol kinase is coupled to an ADP-detecting system has been modified to increase its range (0.008–0.150 μmoles) and reduce the amounts of reagents required. The modified assay was used to assay glyceraldehyde and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride. Mixtures of glycerol with either one of the trioses are differentiated by this method provided that the triose-to-glycerol ratio does not exceed about 3:1. The application of this assay method to a yeast strain growing on these substrates is shown.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b80731a38347fb79bf800d25a9993310 https://pubmed.ncbi.nlm.nih.gov/6057108 Zobrazit plný text záznamu