Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography
| Autor: | Jørgen Aagaard Axelsen, Thomas Larsen, Helle Weber Ravn |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Analytical chemistry Sensitivity and Specificity Biochemistry Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Ergosterol Chromatography Ethanol Organic Chemistry Extraction (chemistry) Reproducibility of Results Water General Medicine Sterol Thin-layer chromatography Solvent chemistry Paraffin lipids (amino acids peptides and proteins) Chromatography Thin Layer Densitometry Oils Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography A. 1026:301-304 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2003.10.128 |
| Popis: | A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100% depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected with thin-layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng. |
| Databáze: | OpenAIRE |
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