Physiological and Molecular Characterization of the Differential Response of Broccoli (Brassica oleracea var. Italica) Cultivars Reveals Limiting Factors for Broccoli Tolerance to Drought Stress
| Autor: | José Manuel López-Nicolás, Sergio Chevilly, Laura Dolz-Edo, José M. Mulet, Luna Morcillo, Alberto Vilagrosa, Lynne Yenush |
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| Přispěvatelé: | Universidad de Alicante. Departamento de Ecología, CEAM (Centro de Estudios Ambientales del Mediterráneo), Gestión de Ecosistemas y de la Biodiversidad (GEB) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Drought stress
Drought tolerance Primary metabolites Brassica Biology 15.- Proteger restaurar y promover la utilización sostenible de los ecosistemas terrestres gestionar de manera sostenible los bosques combatir la desertificación y detener y revertir la degradación de la tierra y frenar la pérdida de diversidad biológica Article Crop chemistry.chemical_compound parasitic diseases Plant hormones BIOQUIMICA Y BIOLOGIA MOLECULAR 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades 13.- Tomar medidas urgentes para combatir el cambio climático y sus efectos Cultivar Abscisic acid Essential amino acids Methionine 06.- Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todos fungi Broccoli food and beverages Primary metabolite General Chemistry Quinic acid Ecología biology.organism_classification Droughts 02.- Poner fin al hambre conseguir la seguridad alimentaria y una mejor nutrición y promover la agricultura sostenible Plant Leaves 08.- Fomentar el crecimiento económico sostenido inclusivo y sostenible el empleo pleno y productivo y el trabajo decente para todos 12.- Garantizar las pautas de consumo y de producción sostenibles Plant Breeding Horticulture chemistry 01.- Erradicar la pobreza en todas sus formas en todo el mundo Brassica oleracea General Agricultural and Biological Sciences Abscisic Acid |
| Zdroj: | Journal of Agricultural and Food Chemistry RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| Popis: | [EN] Broccoli is a cruciferous crop rich in health-promoting metabolites. Due to several factors, including anthropogenic global warming, aridity is increasing in many cultivation areas. There is a great demand to characterize the drought response of broccoli and use this knowledge to develop new cultivars able to maintain yield under water constraints. The aim of this study is to characterize the drought response at the physiological and molecular level of different broccoli (Brassica oleracea L. var. Italica Plenck) cultivars, previously characterized as drought-sensitive or drought-tolerant. This approach aims to identify different traits, which can constitute limiting factors for drought stress tolerance in broccoli. For this purpose, we have compared several physiological parameters and the complete profiles of amino acids, primary metabolites, hormones, and ions of drought-tolerant and drought-sensitive cultivars under stress and control conditions. We have found that drought-tolerant cultivars presented higher levels of methionine and abscisic acid and lower amounts of urea, quinic acid, and the gluconic acid lactone. Interestingly, we have also found that a drought treatment increases the levels of most essential amino acids in leaves and in florets. Our results have established physiological and molecular traits useful as distinctive markers to predict drought tolerance in broccoli or which could be reliably used for breeding new cultivars adapted to water scarcity. We have also found that a drought treatment increases the content of essential amino acids in broccoli. The authors thank Prof. Angel Maquieira for generously providing the technical equipment required for ion determination. S.C.'s work was funded by Grant FPU19/01977 from the Spanish Ministerio de Universidades. This work was funded by the RTC-2017-6468-2-AR project (APROXIMA-CIONES MOLECULARES PARA INCREMENTAR LA TOLERANCIA A SALINIDAD Y SEQUiA DEL BROCOLI) awarded by the "Agencia estatal de Investigacion". L.M. was supported by the Spanish MICINN (PTA2019-018094). Activities of L.M. and A.V. were funded by the Prometeu program (IMAGINA project, PROMETEU/2019/110). The CEAM foundation is funded by Generalitat Valenciana. The authors thank SAKATA for the generous gift of plant material. The authors would like to acknowledge the IBMCP Metabolomics Platform (UPV-CSIC, Valencia, Spain) for the analysis of primary metabolites/metabolomics analysis. |
| Databáze: | OpenAIRE |
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