Erythrocyte Catalase Activity in More Frequent Microcytic Hypochromic Anemia: Beta-Thalassemia Trait and Iron Deficiency Anemia
| Autor: | Magdalena María Terán, Miryam Emilse Ledesma Achem, Cecilia Jiménez, Blanca Issé, Sandra Stella Lazarte, Maria Eugenia Monaco |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
ESTRES OXIDATIVO
medicine.medical_specialty Hemoglobin electrophoresis Article Subject Anemia Thalassemia medicine.disease_cause Bioinformatics Internal medicine hemic and lymphatic diseases purl.org/becyt/ford/3.2 [https] medicine Diseases of the blood and blood-forming organs chemistry.chemical_classification biology business.industry Hematology GEN HFE medicine.disease Enzyme assay MUTACIONES Endocrinology Enzyme chemistry Iron-deficiency anemia Catalase biology.protein BETA TALASEMIA purl.org/becyt/ford/3 [https] RC633-647.5 business Oxidative stress Research Article |
| Zdroj: | Advances in Hematology CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Advances in Hematology, Vol 2015 (2015) |
| ISSN: | 1687-9104 |
| DOI: | 10.1155/2015/343571 |
| Popis: | Most common microcytic hypochromic anemias are iron deficiency anemia (IDA) and β-thalassemia trait (BTT), in which oxidative stress (OxS) has an essential role. Catalase causes detoxification of H2O2 in cells, and it is an indispensable antioxidant enzyme. The study was designed to measure erythrocyte catalase activity (ECAT) in patients with IDA (10) or BTT (21), to relate it with thalassemia mutation type (β0 or β+) and to compare it with normal subjects (67). Ninety-eight individuals were analyzed since September 2013 to June 2014 in Tucumán, Argentina. Total blood count, hemoglobin electrophoresis at alkaline pH, HbA2, catalase, and iron status were performed. β-thalassemic mutations were determined by real-time PCR. Normal range for ECAT was 70,0-130,0 MU/L. ECAT was increased in 14% (3/21) of BTT subjects and decreased in 40% (4/10) of those with IDA. No significant difference (p=0,245) was shown between normal and BTT groups, while between IDA and normal groups the difference was proved to be significant (p=0,000). In β0 and β+ groups, no significant difference (p=0,359) was observed. An altered ECAT was detected in IDA and BTT. These results will help to clarify how the catalase activity works in these anemia types. Fil: Lazarte, Sandra Stella. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina Fil: Monaco, Maria Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina Fil: Jimenez, Cecilia Laura. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina Fil: Ledesma, Miryam Emilse. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina Fil: Teran, Magdalena María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina Fil: Isse, Blanca Alicia de Los Angeles G.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina |
| Databáze: | OpenAIRE |
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