The protective effect of Canova homeopathic medicine in cyclophosphamide-treated non-human primates
| Autor: | Rommel Rodríguez Burbano, Giovanny R. Pinto, Mariana Ferreira Leal, Edilson Ferreira de Andrade, Paulo Pimentel Assumpção, Lusânia Maria Greggi Antunes, Aline Amaral Imbeloni, Marília de Arruda Cardoso Smith, Ismael Dale Cotrim Guerreiro da Silva, Maria Fernanda Vita Lamarão, José Muñiz, Alexandre Pingarilho Rezende, C. A. Silva |
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| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP), Universidade de São Paulo (USP), Fed Univ Para, Minist Saude, Univ Fed Piaui |
| Rok vydání: | 2011 |
| Předmět: |
Male
Cyclophosphamide Canova Lymphocyte proliferation Pharmacology Biology Cebus apella Toxicology Body weight Lymphocyte Activation medicine Leukocytes Animals Cebus Non-human primates Cell Proliferation Micronucleus Tests Macrophages General Medicine Homeopathy Homeopathic medicine Abnormal wbc Immunology Materia Medica Comet Assay Micronucleus Food Science medicine.drug DNA Damage |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| ISSN: | 1873-6351 0402-3900 |
| Popis: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Canova activates macrophages and indirectly induces lymphocyte proliferation. Here we evaluated the effects of Canova in cyclophosphamide-treated non-human primates.Methods: Twelve Cebus apella were evaluated. Four animals were treated with Canova only. Eight animals were treated with two doses of cyclophosphamide (50 mg/kg) and four of these animals received Canova. Body weight, biochemistry and hematologic analyses were performed for 40 days. Micronucleus and comet assays were performed for the evaluation of DNA damage.Results: We observed that cyclophosphamide induced abnormal WBC count in all animals. However, the group treated with cyclophosphamide plus Canova presented a higher leukocyte count than that which received only cyclophosphamide. Cyclophosphamide induced micronucleus and DNA damage in all animals. the frequency of these alterations was significantly lower in the Canova group than in the group without this medicine.Conclusions: Our results demonstrated that Canova treatment minimizes cyclophosphamide myelotoxicity in C. apella. (C) 2012 Elsevier B.V. All rights reserved. Universidade Federal de São Paulo, Disciplina Genet, Dept Morfol & Genet, BR-04023900 São Paulo, Brazil Univ São Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil Fed Univ Para, Lab Citogenet Humana, Inst Ciencias Biol, BR-66059 Belem, Para, Brazil Universidade Federal de São Paulo, Disciplina Ginecol, Dept Tocoginecol, BR-04039032 São Paulo, Brazil Fed Univ Para, Serv Cirurgia, Hosp Univ Joao de Barros Barreto, BR-66073000 Belem, Para, Brazil Minist Saude, Ctr Nacl Primatas, BR-77030000 Ananindeua, PA, Brazil Univ Fed Piaui, Lab Genet & Biol Mol, BR-64202020 Parnaiba, PI, Brazil Universidade Federal de São Paulo, Disciplina Genet, Dept Morfol & Genet, BR-04023900 São Paulo, Brazil Universidade Federal de São Paulo, Disciplina Ginecol, Dept Tocoginecol, BR-04039032 São Paulo, Brazil CNPq: 550885/2007-2 Web of Science |
| Databáze: | OpenAIRE |
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