Regulation of IL-24 in human oral keratinocytes stimulated with Tannerella forsythia
Autor: | Na-Young Han, Hookeun Lee, Bong-Kyu Choi, Sun‐Jin An, Yeon‐Kyeong Ko |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Microbiology (medical) Keratinocytes medicine.medical_treatment Immunology Inflammation Microbiology 03 medical and health sciences 0302 clinical medicine Forsythia medicine Tannerella forsythia CXCL10 Humans Secretion General Dentistry Mitogen-Activated Protein Kinase Kinases biology Chemistry Interleukin-6 Interleukins 030206 dentistry biology.organism_classification Molecular biology stomatognathic diseases 030104 developmental biology Cytokine medicine.anatomical_structure Cell culture medicine.symptom Keratinocyte Reactive Oxygen Species Protein Binding Signal Transduction |
Zdroj: | Molecular oral microbiologyREFERENCES. 34(5) |
ISSN: | 2041-1014 |
Popis: | Interleukin-24 is a pleiotropic immunoregulatory cytokine and a member of the IL-20R subfamily of the IL-10 family. The aim of this study was to investigate the regulation of IL-24 in the human oral keratinocyte cell line HOK-16B following infection with Tannerella forsythia, a major periodontal pathogen. T. forsythia induced the expression of IL-24 mRNA and the secretion of glycosylated IL-24 in HOK-16B cells. Glycosylation of IL-24 is linked to its solubility and bioavailability. T. forsythia-stimulated reactive oxygen species (ROS) induced the expression of IL-24, which was regulated by IL-6. The ROS inhibitor N-acetylcysteine and MAPK inhibitors significantly reduced the expression of IL-6 and IL-24 induced by T. forsythia. Recombinant human IL-24 significantly enhanced the expression of IL-1α, IL-8, CXCL10, and MCP-1 in HOK-16B cells. Together, these results indicate that ROS, MAPKs, and IL-6 comprise the axis of IL-24 expression in HOK-16B cells stimulated with T. forsythia. Thus, IL-24 may be involved in inflammation in oral keratinocytes. |
Databáze: | OpenAIRE |
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