In Vitro and In Vivo Effects of Norathyriol and Mangiferin on α-Glucosidase
| Autor: | Da Song, Li Xiaoying, Mei-Feng Qi, Yang Xujuan, Liu Yidan, Wang Ping, Zhi-Long Shi, Shang Jianhua, Zhaoxiang Yang, Yun-Yun Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glucose tolerance test medicine.diagnostic_test Article Subject 010405 organic chemistry Chemistry Metabolite Pharmacology 01 natural sciences Biochemistry In vitro 0104 chemical sciences lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology In vivo medicine Carbohydrate loading lcsh:QD415-436 Mangiferin IC50 Acarbose medicine.drug |
| Zdroj: | Biochemistry Research International, Vol 2017 (2017) |
| ISSN: | 2090-2247 |
| DOI: | 10.1155/2017/1206015 |
| Popis: | Norathyriol is a metabolite of mangiferin. Mangiferin has been reported to inhibit α-glucosidase. To the best of our knowledge, no study has been conducted to determine or compare those two compounds on inhibiting α-glucosidase in vitro and in vivo by far. In this study, we determined the inhibitory activity of norathyriol and mangiferin on α-glucosidase in vitro and evaluated their antidiabetic effect in diabetic mice. The results showed that norathyriol inhibited α-glucosidase in a noncompetitive manner with an IC50 value of 3.12 μM, which is more potent than mangiferin (IC50 = 358.54 μM) and positive drug acarbose (IC50 = 479.2 μM) in the zymological experiment. Both of norathyriol and mangiferin caused significant (p<0.05) reduction in fasting blood glucose and the blood glucose levels at two hours after carbohydrate loading and it was interesting that mangiferin and norathyriol can make the decline of the blood glucose earlier than other groups ever including normal group in the starch tolerance test. However, norathyriol and mangiferin did not significantly influence carbohydrate absorption in the glucose tolerance test. Therefore, the antidiabetic effects of norathyriol and mangiferin might be associated with α-glucosidase, and norathyriol was more potent than mangiferin. |
| Databáze: | OpenAIRE |
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