Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats
| Autor: | Joanne Tran, Lina Gao, Beth A. Habecker, William R. Woodward, Suzanne S. Fei, Richard Bayles, Antoinette Olivas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Sympathetic Nervous System Molecular biology Stellate Ganglion Gene Expression Biochemistry Rats Inbred WKY chemistry.chemical_compound 0302 clinical medicine Catecholamines Sequencing techniques Medicine and Health Sciences Metabolites Amines Neurotransmitter Receptor Multidisciplinary Organic Compounds Heart Neurochemistry RNA sequencing Genomics Neurotransmitters 3. Good health Chemistry Physical Sciences Medicine Female Anatomy Transcriptome Analysis Acetylcholine medicine.drug Research Article medicine.medical_specialty Biogenic Amines Tyrosine 3-Monooxygenase Cardiac Ventricles Science Neuropeptide Biology 03 medical and health sciences Neurochemical Sex Factors Internal medicine medicine Genetics Animals Tyrosine hydroxylase Myocardium Organic Chemistry Chemical Compounds Biology and Life Sciences Computational Biology Genome Analysis Hormones Rats Research and analysis methods 030104 developmental biology Endocrinology Biological Tissue Metabolism Molecular biology techniques chemistry Catecholamine Cardiovascular Anatomy Ganglia 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 6, p e0218133 (2019) |
| ISSN: | 1932-6203 |
| Popis: | The stellate ganglia are the predominant source of sympathetic innervation to the heart. Remodeling of sympathetic nerves projecting to the heart has been observed in several cardiovascular diseases, and sympathetic dysfunction contributes to cardiac pathology. Wistar Kyoto rats are a common model for the study of cardiovascular diseases, but we lack a profile of the baseline transcriptomic and neurochemical characteristics of their cardiac sympathetic neurons. Most studies of cardiovascular disease have used male animals only, but in the future both male and female animals will be used for these types of studies; therefore, we sought to characterize the transcriptome of male and female stellate ganglia and to correlate that with catecholamine and acetylcholine content in the heart. We have generated a dataset of baseline RNA expression in male and female Wistar Kyoto rat stellate ganglia using RNA-seq, and have measured neurotransmitter levels in heart and stellate ganglia using HPLC and mass spectrometry. We identified numerous gene expression differences between male and female stellates, including genes encoding important developmental factors, receptors and neuropeptides. Female hearts had significantly higher neurotransmitter content than male hearts; however, no significant differences were detected in expression of the genes encoding neurotransmitter synthetic enzymes. Similarly, no statistically significant differences were identified between the sexes in cardiac tyrosine hydroxylase levels. |
| Databáze: | OpenAIRE |
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