High insecticide resistance in the major malaria vector Anopheles coluzzii in Chad Republic

Autor: Ebai Terence, Amen N. Fadel, Samuel Wanji, Magellan Tchouakui, Charles S. Wondji, Micareme Tchoupo, Murielle J. Wondji, Sulaiman S. Ibrahim, Clement Kerah-Hinzoumbe
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Insecticides
Veterinary medicine
Anopheles gambiae
Resistance
Indoor residual spraying
Insecticide Resistance
chemistry.chemical_compound
0302 clinical medicine
Kdr
Sahel
Insecticide
2. Zero hunger
education.field_of_study
qx_4
biology
lcsh:Public aspects of medicine
General Medicine
Anopheles coluzzii
3. Good health
Infectious Diseases
Female
qx_515
Research Article
medicine.drug
Chad
030231 tropical medicine
Population
Bendiocarb
wa_395
Mosquito Vectors
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Etofenprox
Anopheles
qx_600
parasitic diseases
medicine
Animals
lcsh:RC109-216
education
Public Health
Environmental and Occupational Health
wa_240
Knockdown resistance
lcsh:RA1-1270
biology.organism_classification
Malaria
030104 developmental biology
Deltamethrin
chemistry
Metabolic
Permethrin
Zdroj: Infectious Diseases of Poverty, Vol 8, Iss 1, Pp 1-12 (2019)
Infectious Diseases of Poverty
ISSN: 2049-9957
Popis: BackgroundThe Sahel region of Chad Republic is a prime candidate for malaria pre-elimination. To facilitate pre-elimination efforts in this region, two populations ofAnopheles coluzziifrom Central Chad Republic were characterized, their insecticide resistance profile and the possible molecular mechanisms driving the resistance in the field investigated.MethodsBloodfed femaleAnopheles gambiaes.l. resting indoor, were collected at N’djamena and Massakory, Chad in 2018 and characterized for species composition, and infection rate was determined using the TaqMan assay. Susceptibility to various insecticides was assessed using WHO tube bioassays. Cone bioassays were conducted using various long-lasting insecticidal nets (LLINs). Results were analysed using Chi Square test. Knockdown resistance (kdr) andace-1markers were investigated by TaqMan genotyping.ResultsAnopheles coluzziiwas the major vector found in N’djamena (100%) and Massakory (~ 94%). NoPlasmodiumwas found in 147 bloodfed F0An. coluzzii(82 from N’djamena and 65 from Massakory).High intensity pyrethroid resistance was observed with mortalities of χ2 = 22.8, df = 1,P = 0.0006; mortality = 13.0% for deltamethrin,χ2 = 8.8, df = 1,P ace-1mutation points to the role of metabolic resistance in the bendiocarb resistance. Both 1014F and 1014S mutations were found in both populations at around 60% and ConclusionsMultiple resistance inAn. coluzziipopulations from Chad highlight challenges associated with deployment of LLINs and indoor residual spraying (IRS) in the Sahel of this country. The pyrethroid-synergists LLINs (e.g. PermaNet®3.0) and organophosphate-based IRS maybe the alternatives for malaria control in this region.
Databáze: OpenAIRE
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