Newcastle disease virus vectors expressing consensus sequence of the H7 HA protein protect broiler chickens and turkeys against highly pathogenic H7N8 virus
Autor: | Shin-Hee Kim, Ishita Roy Chowdhury, Brian G. Pierce, Siba K. Samal, Sai Goutham Reddy Yeddula |
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Rok vydání: | 2019 |
Předmět: |
Turkeys
animal structures animal diseases viruses Genetic Vectors 030231 tropical medicine Immunization Secondary Newcastle disease virus Hemagglutinin Glycoproteins Influenza Virus Serogroup medicine.disease_cause Newcastle disease Virus 03 medical and health sciences 0302 clinical medicine Consensus Sequence medicine Animals 030212 general & internal medicine HN Protein Vector (molecular biology) Poultry Diseases General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Outbreak biology.organism_classification Virology Influenza A virus subtype H5N1 Vaccination Infectious Diseases Immunization Influenza A virus Influenza Vaccines Influenza in Birds Molecular Medicine Chickens |
Zdroj: | Vaccine. 37:4956-4962 |
ISSN: | 0264-410X |
Popis: | Continuous outbreaks of highly pathogenic avian influenza (HPAI) viruses in commercial poultry have caused devastating losses to domestic poultry with a raising public health concern. The outbreaks of HPAI viruses have increased worldwide, including the North America. Therefore, vaccination has been considered as an alternative strategy for an efficient control of HPAI viruses. In this study, we aimed to generate Newcastle disease virus (NDV) vectored H7 serotype-specific vaccines by expressing the consensus sequence of the HA protein. Conventional NDV strain LaSota vector and a chimeric NDV vector containing the avian paramyxovirus type-2 F and HN protein were able to express the consensus sequence of HA protein. The protective efficacy of vaccines was evaluated in broiler chickens and in turkeys. One-day-old poults were prime immunized with the chimeric vector expressing the HA protein followed by boost immunization with LaSota vector expressing the HA protein or co-expressing the HA and NA proteins. Our vaccine candidates provided complete protection of broiler chickens from mortality and shedding of H7N8 HPAI challenge virus. Turkeys were better protected by boosting with the LaSota vector co-expressing the HA and NA proteins than the LaSota vector expressing only the HA protein. Our study demonstrated a potential use of heterologous prime and boost vaccination strategy to protect poultry against H7 HPAI viruses. |
Databáze: | OpenAIRE |
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