Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
| Autor: | Cheol Hwan Myung, Jae Sung Hwang, Jong Il Park, Ji Eun Lee, Chan Song Jo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Agonist
Cell biology Hydrocortisone medicine.drug_class Molecular biology Science Chromosomal translocation Models Biological Dexamethasone Article Glucocorticoid receptor Receptors Glucocorticoid Gene expression medicine Animals Promoter Regions Genetic Gene Adaptor Proteins Signal Transducing Cell Nucleus Multidisciplinary Melanosomes Base Sequence Chemistry Biological Transport Up-Regulation Mice Inbred C57BL Cytosol Gene Expression Regulation Melanosome transport Melanophilin Medicine Protein Binding |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Mlph plays a crucial role in regulating skin pigmentation through the melanosome transport process. Although Mlph is a major component involved in melanosome transport, the mechanism that regulates the expression of the Mlph gene has not been identified. In this study, we demonstrate that Mlph expression is regulated by the glucocorticoid receptor (GR). Alteration of GR activity using a specific GR agonist or antagonist only regulated the expression of Mlph among the 3 key melanosome transport proteins. Translocation of GR from the cytosol into the nucleus following Dex treatment was confirmed by separating the cytosol and nuclear fractions and by immunofluorescence staining. In ChIP assays, Dex induced GR binding to the Mlph promoter and we determined that Dex induced the GR binding motif on the Mlph promoter. Our findings contribute to understanding the regulation of Mlph expression and to the novel role of GR in Mlph gene expression. |
| Databáze: | OpenAIRE |
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