Proximity assays for sensitive quantification of proteins
| Autor: | Stephen A. Bustin, Gemma Johnson, Sara Kirvell, Christina Greenwood, Harvinder S. Dhillon, David Ruff |
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| Rok vydání: | 2015 |
| Předmět: |
In situ
In situ assays Proximity extension assay Aptamer lcsh:QR1-502 Proximity ligation assay Computational biology Review Article Biology Biochemistry Protein expression lcsh:Microbiology chemistry.chemical_compound Structural Biology Immunoassays Molecular Biology lcsh:QH301-705.5 Immuno pcr Protein molecules Molecular biology chemistry lcsh:Biology (General) Homogeneous Molecular Medicine Immuno-PCR DNA |
| Zdroj: | Biomolecular Detection and Quantification Biomolecular Detection and Quantification, Vol 4, Iss C, Pp 10-16 (2015) |
| ISSN: | 2214-7535 |
| Popis: | Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein–protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression. |
| Databáze: | OpenAIRE |
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