Effects of a High Fat Diet and Voluntary Wheel Running Exercise on Cidea and Cidec Expression in Liver and Adipose Tissue of Mice

Autor: Vishwajeet Puri, Sandrine Couldwell, Alexandra Z. Sosinsky, Vishva M. Sharma, Sayani Banerjee, Allegra Robinson, Jacob Donohue, Thomas H. Reynolds, Ashton Frulla
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
medicine.medical_specialty
medicine.medical_treatment
lcsh:Medicine
Peroxisome proliferator-activated receptor
Adipose tissue
Type 2 diabetes
Biology
Diet
High-Fat
Running
Pathogenesis
03 medical and health sciences
Mice
0302 clinical medicine
Insulin resistance
Internal medicine
Physical Conditioning
Animal
medicine
Animals
Insulin
RNA
Messenger
lcsh:Science
030304 developmental biology
Adiposity
2. Zero hunger
chemistry.chemical_classification
PRDM16
0303 health sciences
Multidisciplinary
lcsh:R
Proteins
medicine.disease
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Mice
Inbred C57BL
PPAR gamma
Endocrinology
chemistry
Adipose Tissue
Gene Expression Regulation
Liver
lcsh:Q
Steatosis
Apoptosis Regulatory Proteins
030217 neurology & neurosurgery
Research Article
Transcription Factors
Zdroj: PLoS ONE
PLoS ONE, Vol 10, Iss 7, p e0130259 (2015)
ISSN: 1932-6203
Popis: Cidea and Cidec play an important role in regulating triglyceride storage in liver and adipose tissue. It is not known if the Cidea and Cidec genes respond to a high fat diet (HFD) or exercise training, two interventions that alter lipid storage. The purpose of the present study was to determine the effect of a HFD and voluntary wheel running (WR) on Cidea and Cidec mRNA and protein expression in adipose tissue and liver of mice. A HFD promoted a significant increase in Cidea and Cidec mRNA levels in adipose tissue and liver. The increase in Cidea and Cidec mRNAs in adipose tissue and liver in response to a HFD was prevented by WR. Similar to the changes in Cidea mRNA, Cidea protein levels in adipose tissue significantly increased in response to a HFD, a process that was, again, prevented by WR. However, in adipose tissue the changes in Cidec mRNA did not correspond to the changes in Cidec protein levels, as a HFD decreased Cidec protein abundance. Interestingly, in adipose tissue Cidea protein expression was significantly related to body weight (R=.725), epididymal adipose tissue (EWAT) mass (R=.475) and insulin resistance (R=.706), whereas Cidec protein expression was inversely related to body weight (R=-.787), EWAT mass (R=-.706), and insulin resistance (R=-.679). Similar to adipose tissue, Cidea protein expression in liver was significantly related to body weight (R=.660), EWAT mass (R=.468), and insulin resistance (R=.599); however, unlike adipose tissue, Cidec protein levels in liver were not related to body weight or EWAT mass and only moderately associated with insulin resistance (R=-.422, P=0.051). Overall, our findings indicate that Cidea is highly associated with adiposity and insulin resistance, whereas Cidec is related to insulin sensitivity. The present study suggests that Cide proteins might play an important functional role in the development of obesity, hepatic steatosis, as well as the pathogenesis of type 2 diabetes.
Databáze: OpenAIRE
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