Comparative Evaluation of qnrA, qnrB, and qnrS Genes in Enterobacteriaceae Ciprofloxacin-Resistant Cases, in Swine Units and a Hospital from Western Romania
| Autor: | Delia Muntean, Mihai Mitulețu, Marius Boldea, J. Dégi, Romeo Teodor Cristina, Florin Muselin, Alexandru O. Doma, Nikola Puvača, Isidora Radulov, Eugenia Dumitrescu, Roxana Popescu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Enterobacteiaceae medicine.drug_class 030106 microbiology Antibiotics qnrB Biology medicine.disease_cause Biochemistry Microbiology law.invention 03 medical and health sciences qnrA Plasmid Antibiotic resistance law medicine Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics genes Gene Escherichia coli Polymerase chain reaction ciprofloxacin-resistant qnrS lcsh:RM1-950 biology.organism_classification Enterobacteriaceae Ciprofloxacin 030104 developmental biology Infectious Diseases lcsh:Therapeutics. Pharmacology medicine.drug |
| Zdroj: | Antibiotics, Vol 9, Iss 698, p 698 (2020) Antibiotics Volume 9 Issue 10 |
| ISSN: | 2079-6382 |
| Popis: | Excessive use of antimicrobials and inadequate infection control practices has turned antimicrobial resistance (AMR) into a global, public health peril. We studied the expression of qnrA, qnrB, and qnrS plasmid in ciprofloxacin (CIP)-resistant strains of Escherichia coli in swine and humans from Romania, using the Polymerase Chain Reaction (PCR) technique. Antibiotic Susceptibility Testing (AST) for human subjects (H) on 147 samples and 53 swine (S) was ascertained as well as the isolation of bacterial DNA (E. coli) as follows: bacteriolysis, DNA-binding, rinsing, elution, amplification, and nucleic acids&rsquo migration and U.V. visualization stages. From 24 samples of E. coli resistant to CIP collected from H subjects and 15 from S, for PCR analysis, 15 H and 12 S were used, with DNA purity of 1.8. The statistically analyzed results using the Crosstabs function (IBM SPSS Statistics-Ver. 2.1.), revealed the qnrS (417 bp) gene in 13 human subjects (52.0%), as well as in all swine samples studied. The qnrB (526 bp) gene was exposed in 9 of the human patients (36.0%) and in all swine isolates, and the qnrA (516 bp) gene was observed only in 3 of the isolates obtained from human subjects (12.0%) and was not discovered in pigs (p > 0.05). The presence of plasmids qnrA, qnrB, and qnrS in the human samples and of qnrB and qnrS in swine, facilitates the survival of pathogens despite the CIP action. The long-term use of CIP could cause a boost in the prevalence of qnr resistance genes, and resistance in the pigs destined for slaughter, a perturbing fact for public health and the human consumer. |
| Databáze: | OpenAIRE |
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